|
KMID : 0613820070170010039
|
|
Journal of Life Science
2007 Volume.17 No. 1 p.39 ~ p.44
|
|
|
Isolation and Characterization of DNA Damaging Agent Sensitivity of rqh1 mutant from Schizosaccharomyce pombe
|
|
Lee In-Hye
Choi In-Soon
|
|
|
Abstract
|
|
|
|
The Rqh1 gene is essential for vegetative growth in fission Yeast. The rqh1 mutant showed that sensitivityof DNA damaging agent, a wild range of phenotype including abnormal gene expression and cell elongation. This result showed that the rqh1-overexpression cell was sensitivity to DNA damaging agent like rqh1 mutant. When Rqh1 have an over-expression by nmt1? promoter of pREP vector, rqh1 mutant DNA damaging agent sensitivity could be compensated. We isolated two strong mutant containing complementation gene, rqh156 and rqh172, respectively. This result observed that the DNA damaging agent sensitivity of rqh1 mutant was complemented by the expression of rqh156 and rqh172. They induced mRNA expression in a dose-dependent manner HU, MMS and UV. The HU sensitivity of the rqh1 was complemented by the expression of rqh156 and rqh172. The mRNA expression of rqh156 decreased on HU dose dependent but the mRNA expression of rqh172 did not decrease on HU dose dependent. The MMS and UV sensitivity of the rqh1 was complemented by the expression of rqh156 and rqh172. These results indicate that the isolated rqh1 gene may play an important role in DNA metabolism.
|
|
|
KEYWORD
|
|
|
RecQ Helicase Family, rqh1?, Hydroxyurea, Methyl-Methan Sulfonate, Ultraviolet
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|